Author summary This research reveals how cells produce 1,5-InsP8, a key molecule in phosphate homeostasis, a vital part of cellular metabolism. Using isotope tracing and mathematical modelling, the study shows that both yeast and human cancer cells follow a linear, rather than branching, pathway to build this molecule. The analysis identified essential steps in the process, challenging previous assumptions about how these molecules are synthesized. By improving our understanding of inositol pyrophosphate metabolism, this work offers new insights into how cells regulate phosphate levels, with implications for cellular energy supply and metabolism in general.
Yeast
In the yeast dataset, the levels of 1-InsP7 were not shown because of their low abundancein the samples [52]. For that reason, we reinvestigated the 18O labelling of IPPs under the high conditions in the cytosol, which favours the accumulation … [17991 chars]
Source: PLOS (Public Library of Science) | Published: 2025-11-10T00:00:00Z
Credit: PLOS (Public Library of Science)
